In a tweet, the President said that his administration will not grant the tech-giant any relief on Mac Pro parts made in China.
The tariffs are 25% on certain parts, which means that customers might be footing the bill.
For years, the president has tried to press the tech company to make more of its products in the U.S. Apple had previously assembled its Mac Pro in the U.S. but made a decision to shift production to China, The Wall Street Journal reported in June.
"Final assembly is only one part of the manufacturing process", Apple said in a statement at the time. Production for the new Mac Pro is said to be taking place in China because that's the only source of certain proprietary components.
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The reaction probably would have been different had Trump made it clear that the tariffs will be applied to Apple's top-selling product, the iPhone, which has always been assembled in China.
Trump has championed companies making products in the US.
Apple hasn't commented on the tweet.
Apple CEO Tim Cook dinner in December 2013 introduced the business was building the Mac Professional in Texas soon after coming beneath criticism for assembling most of its merchandise in decreased-wage nations.
Apple does not disclose sales figures for its Mac Pro, but analysts do not believe it is a high-volume seller for the company compared to the iPhone. The iPhone maker - whose vast and complex supply chain sees some products assembled in America, while others are put together overseas - has recently sought an exemption to duties imposed on the device. It also said the component "is not strategically important or related to "Made in China 2025" or other Chinese industrial programs".