During a press conference after the match, Williams said that Ramos' ruling was "sexist" because she has witnessed male players calling umpires "several things", but they were not penalized. Eventually, Williams called Ramos "a thief", drawing the third violation for "verbal abuse" - and costing her a game, putting Osaka ahead 5-3.
After Ramos' interjection, an angry Williams demanded an apology, even remonstarting "I have never cheated in my life, I have a daughter and I stand what's right for her...", still referring to the first violation.
Earlier, as Williams pleaded her case on court with tournament referee Brian Earley, calling the penalties unfair, she said: "Because you're a woman, you're going to take this away from me?"
From her loss comes a major win for 20-year-old Osaka, a New York-raised Haitian-Japanese woman whose rise she herself says would never have happened if it weren't for her "idol" Serena Williams. This is not fair. The multiple grand slam victor Victoria Azarenka said: "If it was a men's match, this wouldn't happen like this. More voices are needed to do the same", she said.
"Then he got a little tacky when he then accused all coaches ... he kind of went off the rails a little bit but he definitely copped to it that it always happens".
William's French coach, Patrick Mouratoglou, admitted to ESPN he had indeed been giving Williams coaching instructions, but believed Williams had not heard them as she had not looked in his direction.
The former world number one had her second chance of the year to equal Margaret Court's long-standing grand slam record of 24 grand slams, but let her emotions get the better of her against a brilliant and calm Naomi Osaka in NY. "I know you guys were here rooting, but let's make this the best moment we can".
Japan hails Osaka, Nishikori's historic run at US Open
Until Osaka's run this year, the best NY performance by a Japanese woman was 2004 when Shinobu Asagoe reached the quarterfinals. Aliaksandra Sasnovich , who bested Kvitova at Wimbledon, fared even worse and had to endure a humbling 0-6 0-6 loss.
Serena Williams was docked a game by an umpire after a series of controversial calls. She then snapped her racket in half, resulting in a point violation.
Serena Williams is a Grand Slam champion who is not afraid of making her thoughts known. "Coaching on every point should be allowed in tennis. I don't want to do questions", she said on court.
On Saturday, the 36-year-old tennis star was defeated in the U.S.
"It still feels really weird because I've never beaten Madison before", Osaka said.
"I just feel like the fact that I have to go through this is just an example for the next person that has emotions and that want to express themselves and want to be a strong woman".
Osaka was tearful despite having won the match and remarked she was "sorry it had to end like this". "Maybe in a few days I'll realize what I've done", said Osaka, adding that the noise was so great in Arthur Ashe Stadium and her focus so single-minded that she wasn't fully aware of the escalating controversy.
"An umpire so wrecked a big occasion that both players, Naomi Osaka and Serena Williams alike, wound up distraught with tears streaming down their faces during the trophy presentation and an incensed crowd screamed boos at the court", Ms Jenkins wrote.